Tuesday, January 8, 2013

Study : shoot response to several auxin (IAA, NAA, IBA) concentration single and combined.

Study the dynamic of shoot on different concentration of 3 types of auxin, most active auxin is IBA.  But for in vitro rooting, I must be very cautious to develop a new concentration for rooting medium.  Each clone may have a specific requirement for hormon, too high, as I see in E hybrid clones, may develop callus prior to root, which maeans callus differentiate from callus, which I do not preferred.  Some literature suspect it as not having vascular connection to stem.

some may even develop root from unwanted parts such as leaves, petiole, which I think not unuseful and can break up easily upon washing.

so we need to test each of them, one by one, within a not too wide/large concentration




2 comments:

  1. belum keluar hasilnya, trit 1-1.5 IBA.
    NAA 2 + IBA 1, pake Media 1/2 MS.

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  2. Surprising result when we tested 4 kind of hormon composition using 1/2 MS basal medium.
    NAA 0.8 + IBA 0.8.............85%
    NAA 1.8 + IBA 1.6.............70%
    NAA 2.4 + IBA 2.4..............75%
    NAA 2.4........................85%
    IBA 2.4.........................85%

    An the root is rigorous

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