Clonal Propagation of Mature Trees of Gmelina arborea Roxb. Via in-vitro Technique

Clonal Propagation of Mature Trees of Gmelina arborea Roxb. Via in-vitro Technique

Yogeshwar Mishra, Fatima Shirin

Abstract

In-vitro axillary bud proliferation has been achieved in nodal segment explants from the side branches of 15-18 years old trees of Gmelina arborea on MS medium +10 μM BA. MS medium containing 1.0 μM BA and 0.1 μM Kn induced maximum shoot multiplication. The addition of 23 μM AgNO₃ in the culture facilitated callus free shoot formation. Rooting was achieved on ½ MS medium enriched with 10.0 μM IBA and rooted plantlets were successfully acclimatized in high humidity conditions (80-90% RH) for two weeks prior to successful transfer to a shadehouse. The eight months old potted plants were showing excellent growth and development.