Low cost Tissue Culture

A few days ago, I read an interesting report about, how tissue culture can be made cheap.  This is intersesting since people perception that plant tissue culture requires sophisticated equipment, and require infrastructure to support.  But, it seems to me that, tissue culture, if, has to be creates big impact to agriculture and forestry, it must be easy and low cost.

My first attempt to make tissue culture at low cost will be using available chemical, instead of the highly expensive chemical from chemical factory, such as Merck, Sigma, and Duchefa...those three are common brand for me as researcher, but farmer will not be able to afford that.  So, what could be the alternative ?

Let's look at the MS salt, it consist of

•  KNO3
• MgSO4*7H20
• KH2PO4
• CaCL2
• NH4NO3

And how will I replace them with cheaper chemie :

• KNO3 : there is on the internet chemical like this, KNO3 cap traktor Pak Tani
• MgSO4 *H2O : Can be substitute with Kieserite Fertilizer
• KH2PO4 : on the internet it is avaiable on cheap price
• CaCL2 : it's cheap no need to replace
• NH4NO3 : can replace using ZA fertilizer (NH4)2SO4

How about Vitamin....it can be done using kid's vitamin (it contain a variety of vitamin from B Complex, such as B Combion, vit C from fpharmacist  , riboflavin, biotin et cetera ....various vitamin on the drug store)

How about hormon.....well this I have to say, not to be substitute, we have to purchase that


How about autoclaving ???????

Well, you can use your microwave, magic com, boil it, and can be sterilize prior to autoclaving using Fungicide (eg methyl thiophanate), NaOCl (bayclin, NaDCC, 3 ppm) by mixing it before autoclaving, to ensure potential microbe be eliminated completely.



See you, I'll continiue this report anytime soon


Happy holiday, happy new year