Abstract
In vitro propagation technique ofGmelina arborea
multipurpose and a fast growing tree species was studied. Nodal segment
including axillary bud was used as a explant. They were cultured on MS
media containing various concentrations (0–10 mg/l) of BAP alone or in combination with 0.002 mg/l of IBA. Nodal segments showed axillary bud proliferation in almost all media tested. MS media containing 0.22 mg/l of BAP alone and 2 mg/l of BAP in combination with 0.002 mg/l of IBA were effective for inducing multiple shoots and shoot elongation. MS medium supplemented with 0.02 mg/l of NAA and 1 mg/l
of IBA gave the best result for rooting. The regenerated plantlets were
potted and acclimatized successfully in a growth chamber and then moved
to the green house. Adventitious shoots production from stem explants
that were taken from regenerated plantletin vitro
was also discussed. Stem segments were tested for their morphogenetic
potential on MS media with various combinations and concentrations of
BAP, zeatin and TDZ. Successfull result was obtained on MS media
supplemented with 2 mg/l of BAP and 1 mg/l of zeatin or supplemented with 0.5 mg/l of BAP and 0.5 mg/l of TDZ. The shoots obtained on MS media containing 2 mg/l of BAP and 1 mg/l of zeatin rooted on MS media containing 0.02 mg/l of NAA and 1 mg/l of IBA, and plantlets were successfully obtained.
A part of this paper was presented at the 109th Annual Meeting of the Japanese Forest Society (1998).
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