eucalyptus: 2.4 D and TDZ over dose

Between TDZ and 2.4 D, which one can give best result on callous frequency and shoot regeneration. I got some info that 2.4 D can produce not true to type in high degree, info please help

2 days ago

8 comments

PietUnfollow

Piet van der Linde • Well Freddy, you can say the same for TDZ. It will depend very much on the amount you have to use to get the reaction you want to have and the sensitivity of the specific plant tissues for both growth regulators. In general, 2.4-D won't give much shoot regeneration unless you are looking for somatic embryos. Just try and see what happens.

1 day ago • Like

BoUnfollow

Bo Ouyang • It may also perform different for different plant species.

1 day ago • Like

Follow Lázaro

Lázaro Cabral • For Tissue culture of Phalaenopsis you can be good results with TDZ because the 2,4 D is too mutagenic. But, for the tissue of tree, the 2,4D can be better togheter some auxin.
How the culture you will work ?

19 hours ago • Like

Follow Leonardo

Leonardo Matute Tiffer • that species need that information?
in my experience, the 2.4 D is better than TDZ, TDZ aid in the formation of callus, but not in the high frequency. but also a higher concentration of 2,4-D, you will have more chance of mutations

15 hours ago • Like

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Lázaro Cabral • Dear Leonardo
2,4D coud be good when you put togheter another citokin... But what is important know is : How culture our friend will work ,, what do you think ?

15 hours ago • Like

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Leonardo Matute Tiffer • First you must know you want to work
or will have no practical help

15 hours ago • Like

EaswaramurthyUnfollow

Easwaramurthy Ramasamy • Sorry to say,Freddy,use the word callus in
place of callous and callous is a carbohydrate substance(similar to cellulose)
deposited on sieve elements,very different from your undifferentiated parenchymatic
callus.

14 hours ago • Like

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Kalyani Dias • Hi, First of all 2,4 D is an auxin and TDZ is not. It has more of cytokinine like properties. The auxins are believed to promote callus forming or root initiation when used externally and cytokines are used externally to promote shoot regeneration. The reason I used the words "externally used" is that the internal level of these growth regulators or hormones can have an impact on the level of external growth regulators required and the outcome of the level used. This effect vary from plant species to plant species. In any plant species, over use of any hormone can cause problems. However, this is been done to manipulate some situations dramatically, where other methods are fruitless or less effective. In such situations residual effects of growth regulators should be removed by either growing plants with no hormones or even using a cleansing system like adding activated charcoal to the media. Now, these are the basics. Without knowing the plant system I cant comment on specifics. However, you can apply these to your own situation to come up with whats suitable. Good Luck.

4 hours ago • Like