One of important process in teak tissue culture establishment is surface sterilization prosedures. I try several times, and I hardly get one sterile and viable explants. Most of my explants was contamiminated by fungus,....which I understand as being from open area, which gives a high persistencies of microbe, deep inside the tissues.
On the surface of teak shoot tips, there is some kind of red pigment which becomes visible whenever we rub the skin surface, and in the water the red coloration becomes more visible.
The surface of the explants itself which contains plenty of hairs, added tothe difficulties of the sterilization process.
But, what I miss, and I findly got hope may be is ...the information that I should subculture the explants every 12 hours.
The phenolic compounds inside the explants tissues became toxic to the living tissues, and prolonged exposure will have more dead tissues becomes dead.
so the chemical produce by the tissues has become a poison to the living tissues, and delayed subculture will increase number of cells unable to survive.
I haven't try this technique, but I think there is element of truth behind it.
well, it's time to try another approch.
In every turn, there must be a new scene.
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