eucalyptus: Trichoderma in TC Plant

Trichoderma sp has been using in the crops production to control disease, can anyone share how can it be incorporated on tissue culture medium or else so we can have the benefit as soon as possible

3 days ago

6 comments

EaswaramurthyUnfollow

Easwaramurthy Ramasamy • Several Trichoderma spp are known for their
antagonistic properties against most of the plant pathogenic and free living fungi by
way of both, parasitic and antibiosis.This happens in soil,especially at rhizosphere.
This ability could be studied in lab on plates,but on a tissue culture medium with
slow growing explants,it is impossible,because,this antagonistic fungus with its
fast growth,cover the whole surface within days and thus denying your growing tissues
for space,carbon and other growth sources,
Again this itself become a problem:a worst contamination.

2 days ago • Like

FREDDY PANGARIBUAN Indonesia • yes, I have tested them on my rooting in vitro, and within 4 days the whole surface already green, but I'll plant them in the nursery since the shoot is not dead until now, unlike other fungus, the shoot will probably dead by now.

2 days ago

Follow Murdock Ray

Murdock Ray Gillis • It may be interesting to apply this to the soil mix that the tissue cultures will be planted into. The source of carbon (sugar) is removed in this transplanting step. Therefore, many "contaminants" that would harm the plant in tissue culture due to the sugar serving as an energy source are not as big a factor once in the greenhouse setting.

1 day ago • Like

Follow Leonardo

Leonardo Matute Tiffer • you need to isolate the fungus?
if so, you need a common means of multiplication of fungi. (FDA)
if what you want is the way of application to crops, know the number of colony forming affecting disease you have.

and if it is applied directly to a medium with the in vitro culture, I agree with Mr.Easwaramurthy Ramasamy "It´s Impossible"

1 day ago • Like

Follow Dr. Barry

Dr. Barry Nadel • Adding Trichoderma to the tissue culture media gives you no real advantage, but it does contaminate your culture. Add the Trichoderma to your first potting mix or even in the media you harden off your plantlets. The real problem with Trichoderma in a commercial nursery is that it is quite expensive. It was used in the nursery I work at for 3 years, but the cost was to great.
It does work quite well against root and stem rots. There are other cheaper means of controlling those pathogens.

1 day ago • Unlike