Explants sterilization of Eucalyptus using NaDCC

Sterilising seed with 500ppm NaDCC

by mfdes » Tue Jun 08, 2010 5:47 am

Hi everyone,

I normally disinfest my explants and seed with 5,000 ppm NaDCC, 5-10m for seed, or under 2m for leaf and stalk tissue.
A while ago I was enquiring about using GA3 for recalcitrant seeds. I normally use a 500ppm GA3 solution for 24-48h, followed by the disinfestation protocol above. Someone suggested using a combined solution of 500ppm GA3 AND 500ppm NaDCC for 24h to disinfest my seed.
I know 24h soaks with low concentration disinfestant are used with tissue, but how well do they work on seed? Is it possible to combine GA3 treatment and disinfestation? Will the seeds not imbibe too much NaDCC?
This brings me to the question of, if the imbibed NaDCC is not harmful, why rinse it at all? I have seen it listed as an ingredient in medium used to inhibit microbial growth...

I found NaDCC by accident in the supermarket, with the commercial name of
AQUATABS(TM), developed by Bayer, to be used in disinfection of fruits,
vegetables, water and nursing bottle. It is presented in tablets, whose
contain 167mg of sodium dicholoroisocyanurate.
So, i prepared 1 liter stock solution with 5 tablets. The final
concentration was 0,5% active chlorine, because each tablet has 100mg of
active chlorine (167mg NaDCC)
The medium was prepared diluting (distilled water) 2XMS frozen stock in MS
normal strenght.
I preferred to use Phytagel cause of its clarity to observe contaminants.
pH=5,8. Microwave was used to dissolve gelrite with the amount of medium +
0,01% active chlorine (NaDCC) in the final volume.
After ebullition, it was dispensed in non-sterile glass vessels and wrapped
with alumminium paper. Nothing was sterilized. I forgot the autoclave. It's
ready!!

The inoculation was done under sterile and non-sterile conditions.
Under non-sterile and open-air condition, after the subculture of
aseptically-grown eucalypts explants, the surface of the medium and
explants was sprayed with 0,05%(Cl) solution of the same product.
Up to that time (7 days), no contaminant was observed under both
conditions. I am observing its effect in growth and development of
the plants.
Today, i prepared again this medium, but using agar, and spraying
the vessels, with 70% alcohol, previously. 

Working Smarter, not Harder

Insentif yang diberikan perusahaan secara finansial kepada pekerja sering terabaikan dengan system yang tidak terlalu jelas dalam menuntut kinerja pekerja.
Ada anggapan bahwa pintar goblok gaji sama, seperti di PNS, atau di swasta, masuk kerja jam 7 pulang jam 5, gaji tetap segitu-segitu.
Mungkin yang bisa ditawarkan kepada pekerja adalah agar bekerja lebih smart, dalam arti  bagaimana membuat proses kerja lebih sederhana, dan efisien sehingga dengan beban kerja yang besar dapat diselesaikan dengan metode/managemen yang lebih ramping (lean management).

Tapi saya sering merasa ragu dengan teori efisiensi, karena efisiensi membuat energi yang lebih besar untuk konsentrasi dan konsistensi, yang sulit untuk diaplikasikan.  Akibatnya, efisiensi tercapai, tapi kerja semakin menegangkan.

http://gsm.ucdavis.edu/spotlight-story/working-smarter-not-harder-genentech

Indonesia's Native Species Orchid Restoration and Conservation Project (INSORCP)

Habitat destruction, modification anf fragmentation are serious threat to native orchid
The goal of the Native Species Orchid Restoration and Conservation Project is to protect native orchids of Indonesia through the use of modern propagation science, recovery and translocation methods. A laboratory and associated facilities are located on the refuge. This project will involve and benefit state and Forestry company and  agencies to actively  collect, conserve, restore and protect native orchids in Indonesia. The Sinarmas Forestry Technology Centre (SFTC) is a primary partner in the research of propagation techniques and natural history of Indonesia's native orchids.

Orchid listed below are protected by Law, according to Indonesia's Regulation ( Peraturan Pemerintah Republik Indonesia, No. 7 Tahun 1999, Tanggal 27 Januari 1999)

• Ascocentrum miniatum, Anggrek kebutan
• Coelogyne pandurata, Anggrek hitam
• Corybas fornicatus, Anggrek koribas
• Cymbidium hartinahianum, Anggrek hartinah
• Dendrobium catinecloesum, Anggrek karawai
• Dendrobium d’albertisii, Anggrek albert
• Dendrobium lasianthera, Anggrek stuberi
• Dendrobium macrophyllum, Anggrek jamrud
• Dendrobium ostrinoglossum, Anggrek karawai
• Dendrobium phalaenopsis, Anggrek larat
• Grammatophyllum papuanum, Anggrek raksasa Irian
• Grammatophyllum speciosum, Anggrek tebu
• Macodes petola, Anggrek ki aksara
• Paphiopedilum chamberlainianum, Anggrek kasut kumis
• Paphiopedilum glaucophyllum, Anggrek kasut berbulu
• Paphiopedilum praestans, Anggrek kasut pita
• Paraphalaenopsis denevei, Anggrek bulan bintang
• Paraphalaenopsis laycockii, Anggrek bulan Kaliman Tengah
• Paraphalaenopsis serpentilingua Anggrek bulan Kaliman Barat
• Phalaenopsis amboinensis, Anggrek bulan Ambon
• Phalaenopsis gigantea, Anggrek bulan raksasa
• Phalaenopsis sumatrana, Anggrek bulan Sumatera
• Phalaenopsis violacose, Anggrek kelip
• Renanthera matutina, Anggrek jingga
• Spathoglottis zurea, Anggrek sendok
• Vanda celebica, Vanda mungil Minahasa
• Vanda hookeriana, Vanda pensil
• Vanda pumila, Vanda mini
• Vanda sumatrana, Vanda Sumatera

Daftar ini diambil dari Situs Departemen Kehutanan RI.